Generator

Part:BBa_K1060009:Design

Designed by: Ingmar Claes, Su Wang, Sylvie Weckx   Group: iGEM13_KU_Leuven   (2013-09-19)


Medium constitutive expression of EBF synthase from Artemisia annua


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 177
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1368
    Illegal BamHI site found at 677
    Illegal XhoI site found at 639
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 249


Design Notes

no EcoRI, XbaI, NotI, SpeI or PstI sites present


Source

Composite part; genomic sequence from EBF synthase from Artemisia annua combined with iGEM bricks. BBa_K608006 + Bba_K1060002 + Bba_B0015

References